Journal: Microbiology Spectrum

Article Title: 6-Thioguanine Inhibits Herpes Simplex Virus 1 Infection of Eyes

doi: 10.1128/Spectrum.00646-21

Figure Lengend Snippet: Details of antibodies used in this project

Article Snippet: Anti-ICP4 (H943) antibody , Santa Cruz , SC-69809 (lot no. J1520) , 1:500 , 1:100.

Techniques: Western Blot, Immunofluorescence

Journal: Virologica Sinica

Article Title: Nuclear translocation of Sp100A suppresses multiple DNA viruses and limits HSV-1 lytic replication in vivo

doi: 10.1016/j.virs.2026.02.003

Figure Lengend Snippet: Modulation of viral replication by Sp100A mutations on HSV-1. A HEp-2 cells were mock infected, or with the indicated viruses at an MOI of 5 for 6 h. Protein expression was evaluated by immunoblotting with anti-Flag, anti-β-actin, anti-TK, anti-ICP0, and anti-ICP8 antibodies. Note that the target proteins (Flag, TK, ICP0, and ICP8) were detected on separate blots. β-actin was used as a loading control and was run on a parallel blot using the same batch of protein lysates and identical loading amounts to confirm equal sample loading. B, C Growth kinetics of the indicated viruses in HEp-2 cells at MOIs of 0.01 ( B ) and 5 ( C ). D, E Growth kinetics of the indicated viruses in Vero cells at MOIs of 0.01 ( D ) and 5 ( E ). Data in B – E , statistical comparisons of viral titers were performed between group rHSV-1 and groups rHSV-1-A, rHSV-1-A S188A and rHSV-1-A S188D , as well as between group rHSV-1-A S188A and groups rHSV-1-A and rHSV-1-A S188D . Statistical analyses were performed using two-way ANOVA. F Plaque size comparison of the indicated viruses in HEp-2 and Vero cells. G, H Plaque size of each virus was quantified from 50 plaques using ImageJ and plotted. The data were normalized to the mean plaque size of the rHSV-1-A S188A group. Statistical analyses were performed using one-way ANOVA. Data presented as mean ± SD. ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Antibodies and drugs used in this study included the following: rabbit polyclonal anti-Flag antibody (Catalog No. 80010, Proteintech, USA), rabbit polyclonal anti-Sp100 antibody (Catalog No. GTX131569, GeneTex, USA), mouse monoclonal anti-Flag antibody (Catalog No. AB0008; Abways, Beijing, China), mouse monoclonal anti-β-actin antibody (Catalog No. 1000166; Sino Biological, Beijing, China), rabbit monoclonal anti-GAPDH antibody (Catalog No. AB0037, Abways Technology, Beijing, China), mouse monoclonal anti-Histone H3 antibody (Catalog No. 100005-MM01, Sino Biological, Beijing, China), rabbit polyclonal anti-TSG101 antibody (Catalog No. 28283-1-AP; Proteintech; USA), mouse monoclonal anti-calnexin antibody (Catalog No. sc23954, Santa Cruz, USA), mouse monoclonal anti-ICP0 antibody (Catalog No. sc53070, Santa Cruz, USA), mouse monoclonal anti-ICP8 antibody (Catalog No. ab20194, Abcam, UK), rabbit polyclonal anti-TK antibody (custom-made, GenicBio Limited, Shanghai, China), Alexa Fluor 594-labeled goat anti-rabbit IgG (H + L) (Catalog No. 2165334, Invitrogen, USA), goat anti-mouse IgG-HRP (Catalog No. 31430, Invitrogen, USA), goat anti-rabbit IgG (H + L)-HRP (Catalog No. 32460, Invitrogen, USA), and a protease inhibitor cocktail (Catalog No. EO0492, Thermo Scientific, USA).

Techniques: Infection, Expressing, Western Blot, Control, Comparison, Virus

Journal:

Article Title: Herpes Simplex Virus Type 2 Glycoprotein E Is Required for Efficient Virus Spread from Epithelial Cells to Neurons and for Targeting Viral Proteins from the Neuron Cell Body into Axons

doi: 10.1016/j.virol.2010.06.006

Figure Lengend Snippet: HSV-2 gE is required for targeting envelop and capsid proteins into axons. SCG neurons were infected with 105 PFU of WT, rgE2-del or gE2-del virus and at 18 h post infection, neurons were stained with antibodies to viral antigens gD (red, first column), VP26 (red, fourth column), to SCG neurofilaments (green, second and fifth columns), and as merged images (third and sixth columns). White arrows indicate viral antigens in axons. Most cells are infected as separate neurons; however, some neurons are in clusters (infected with WT or rgE2-del virus and stained for VP26). Note the total absence of gD or VP26 antigen (red) in SCG axons infected with gE2-del virus. Magnification is 400X.

Article Snippet: Polyclonal rabbit anti-HSV-1 VP26 was provided by Prashant Desai, Johns Hopkins University.

Techniques: Infection, Virus, Staining